Interpretation is made in accordance to the guidelines laid down in the nccls m100s12. Mycobacterium tuberculosis drug susceptibility testing. These changes apply to penicillins, cephalosporins and monobactams, and are. Carbapenem resistances in enterobacteriaceae, can commercial. Quality control in antimicrobial susceptibility testing 29 8. Progress in antifungal susceptibility testing of candida spp. The subcommittee has worked diligently to improve m31a2performance standards for antimicrobial disk and dilution. This document provides updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02a11, m07a9, and m11. Areas of technical uncertainties atu and information on how to handle unavoidable technical uncertainty in antimicrobial susceptibility testing. Performance standards for antimicrobial susceptibility testing, 24th informational supplement 24th edition. European committee on antimicrobial susceptibility testing 2012. The clinical and laboratory standards institute clsi subcommittee on antimicrobial susceptibility testing has recognized that many published studies reporting the performance of commercial asts casts suffer from major design andor analysis flaws, rendering the results. Twentieth informational supplement on free shipping on qualified orders.
Antimicrobial sensitivity tests, control and prevention. Performance of standards for antimicrobial susceptibility testing ast clsi 2016 presented by. Clsi methods development and standardization working group. Hakemivala associate prof of sbmu in the name of god panel of. M100s performance standards for antimicrobial susceptibility testing this document provides updated tables for the clinical and laboratory standards institute. Application of computers in antimicrobial susceptibility testing 39 10. The staphylococcus aureus were tested for methicillin susceptibility using 5.
Approved standard third edition describes the standard agar disk diffusion method, as well as standard. The colony counts of the inoculum were performed on drugfree agar medium and. Munro, cls, mt ascp ast qc is a critical component of a quality control plan qcp to ensure reliable patient results. Atu and information on how to handle unavoidable technical uncertainty in. Antimicrobial susceptibility testing of bacteria isolated from aquatic animals and their environments. Dillon, b vanessa allen, c brigitte lefebvre, d linda hoang, e greg tyrrell, f paul van caeseele, g paul levett, h and irene martin a. Direct antimicrobial susceptibility testing of positive. June 2010, january 2011, january 2012, january 20, january 2014, january 2015. Appropriately performed qc procedures monitor a test systems precision i. Laboratory standards institute antimicrobial susceptibility testing standards m02a12. Silley 2012 has argued that the terms resistant and sensitive should. Performance standards for antimicrobial disk susceptibility tests, th edition view sample pages this standard covers the current recommended methods for disk susceptibility testing and criteria for quality control testing.
Performance standards for antimicrobial susceptibility testing, twentyninth edition, m100ed29e. Susceptibility testing approved these mic qc ranges in january 2012 and disk. Instituterecommended methods for disk susceptibility testing, criteria for. Clsi m07ed11 and m100ed29 package methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, eleventh edition, m07ed11e. Quality assurance for antimicrobial susceptibility testing. Assessment of the phoenix automated system and eucast. This document includes updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02, m07, and m11. Testing for these drugs is done using the kirby bauer method at this time, since they are not yet available for testing using the broth microdilution method. Eucast breakpoint criteria are being adopted by automatic antimicrobial susceptibility testing systems.
Results are reported as the mic in micrograms or unitsml. Data from the canadian national gonococcal antimicrobial susceptibility comparison program, including results from 25 proficiency panels distributed between 2003 and 2012, were analyzed. Standards institute antimicrobial susceptibility testing standards. In june 2010, the clinical and laboratory standards institute clsi published new mic and disk diffusion interpretive criteria i. Subcommittee on antimicrobial susceptibility testing since 2012. Clinical and laboratory standards institute document m02a11performance standards for antimicrobial disk susceptibility tests. The clsi publication, performance standards for antimicrobial disk susceptibility tests. Effective evaluations of antimicrobial susceptibility tests asts require robust study design.
Setting male and female std clinic of regional std. Antifungal susceptibility testing, ul standards pdf, hei standards, engine performance pdf, performance metrics pdf, performanceflatplatevsvacuumtube. Of particular importance is the concept of efficacy ratios. A clinical and laboratory standards institute clsi m23style quality control qc. M02a11, performance standards for antimicrobial disk susceptibility tests. Twentysecond informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute clsiapproved standards. Performance standards for antimicrobial disk susceptibility tests, th edition view sample pages this standard covers the current recommended methods for disk susceptibility testing and. United states department of health and human services. Use of trade names and commercial sources is for identification only and does not imply. M02ed performance standards for antimicrobial disk. Susceptibility tests for bacteria isolated from animals.
Quality assurance for antimicrobial susceptibility testing of. In this document, we attempt to answer many of the frequently asked questions regarding antimicrobial susceptibility testing. Comparative assessment of cds, clsi disc diffusion and etest. Educational susceptibility testing as a critical component of. Twentysecond informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory. Quality assurance for antimicrobial susceptibility testing of neisseria gonorrhoeae in canada, 2003 to 2012 pam sawatzky, a gary liu, a joanne r. The clinical importance of antimicrobial susceptibility test results requires that these tests be. Performance standards for antimicrobial susceptibility testing clsi.
Twentysecond informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute. Antimicrobial susceptibility testing for many years, and she is a member of two clsi working. Approved standardeleventh edition includes a series of. Twelfth informational supplement mic interpretative standards as. The clinical and laboratory standards institute clsi and the european committee on antimicrobial susceptibility testing eucast recently changed their recommendations concerning the interpretation and reporting of in vitro drug susceptibility testing dst results. Standard methods for the detection of antimicrobial resistance.
The clinical and laboratory standards institute clsi subcommittee on antimicrobial. Performance standards for antimicrobial susceptibility testing this document includes updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02, m07, and m11. These tests can be useful for screening isolates to categorize them as susceptible, intermediate, resistant, or non. Ampicillin, coamoxiclav, cefuroxime and cefaclor minimum inhibitory concentrations mics were determined by etest on haemophilus test medium. There are two internationally recognized standard methods for the performance of antifungal susceptibility testing of candida spp. Clinical breakpoints fungi candida and aspergillus guidance for organisms not listed in breakpoint tables. Performance standards for antimicrobial disk susceptibility tests. Twentythird informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute. The clinical laboratory standards institute clsi and the european committee on antimicrobial susceptibility testing eucast guidelines are the most popular breakpoint guidelines used in antimicrobial susceptibility testing worldwide. However, investigators must remain cognizant that the performance characteristics of. Performance standards for antimicrobial susceptibility testing 2001 ferraro, mary jane.
Comparison of clinical laboratory standards institute and. Performance standards for antimicrobial susceptibility testing. Purpose the purpose of the kirbybauer disk diffusion susceptibility test is to. Twentyfourth informational supplement abstract the supplemental information presented in this document is. Antimicrobial susceptibility of invasive haemophilus influenzae, 2012 the antimicrobial susceptibility of all 60 viable invasive isolates of h.
Apr 11, 2016 the clinical laboratory standards institute clsi and the european committee on antimicrobial susceptibility testing eucast guidelines are the most popular breakpoint guidelines used in antimicrobial susceptibility testing worldwide. Laboratory standards institute antimicrobial susceptibility testing. This document provides updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02a11, m07a9, and m11a8. M100 performance standards for antimicrobial susceptibility testing, 30th edition. The average mic agreement between the participating laboratories ranged from 85. Educational susceptibility testing as a critical component. American proficiency institute 2012 1st test event antimicrobial susceptibility testing ungraded participants were asked to perform antimicrobial susceptibility testing on this k. International standards organisation susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices. The importance of quality control qc for antimicrobial. Performance standards for antimicrobial susceptibility. Etest is a quantitative technique for determining the antimicrobial susceptibility of gramnegative and grampositive aerobic bacteria such as.
Laboratory methodologies for bacterial antimicrobial susceptibility testing 4 oie terrestrial manual 2012 i disk diffusion, ii broth dilution, iii agar dilution. Determination of disk diffusion and mic quality control guidelines. Comparative assessment of cds, clsi disc diffusion and. National committee for clinical laboratory standards corporate author.
Progress in antifungal susceptibility testing of candida. Approved standard 9th edition, represents the standard for clinical laboratories performing susceptibility testing today. See also the eucast aminoglycoside guidance document for the use of the revised aminoglycoside breakpoints. Kirbybauer disk diffusion susceptibility test protocol. Errors in interpretation and reporting results 28 7. Background a variety of techniques are available for antimicrobial susceptibility testing of neisseria gonorrhoeae. Twentyfifth informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute. The performance of antimicrobial susceptibility testing programmes. Mycobacterium tuberculosis drug susceptibility testing program. Objective the aim of this study was to find a costeffective, reliable and easily applicable microbiological method to detect antimicrobial susceptibilities of n. Buy performance standards for antimicrobial susceptibility testing. Performance standards for antimicrobial disk and dilution. Susceptibility testing of fastidious bacteria 21 6.
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